Prevalence of MDR and Colistin resistant Klebsiella Pneumoniae clinical isolates- A study from western India.

• Savita Umratkar,  
• Sunita Mangesh Bhatawadekar,  
• Bharati Avinash Dalal*

Abstract

Introduction: Klebsiella pneumoniae is an important cause of nosocomial and community-acquired Gram-negative bacteraemia; it causes pneumonia, urinary tract infections and surgical site infections worldwide. Multidrug resistant (MDR) K. pneumoniae has become a major increasing serious public health threat around the world due to the dearth of alternative existing antibiotics. Aim & objectives: The present study was undertaken to find out the prevalence of multi drug resistant (MDR) K. pneumoniae, with objectives to isolate, identify the Klebsiella pneumoniae from clinical samples, to determine antibiotic resistance pattern of Klebsiella pneumoniae isolates and to study colistin susceptibility of Klebsiella pneumoniae isolates by broth micro dilution method. Materials and Methods: Sputum, broncho alveolar lavage (BAL), blood, urine, pus and cerebrospinal fluid (CSF) samples were processed by standard microbiological techniques. Klebsiella pneumoniae was identified by manual as well as by automated method (VITEK-2). Antibiotic susceptibility was tested by automated system (VITEK 2, bioMérieux). Colistin minimum inhibitory concentration (MIC) detection was done by broth micro dilution method. Results: Out of 263 K. pneumoniae isolates, 119(45.24%) were carbapenem resistant(CR) and 55(20.91%) were only extended spectrum beta lactamase (ESBL) producer. 16(6.08%)isolates were colistin resistant. Conclusion: Increasing prevalence of MDR Klebsiella pneumoniae could pose a real problem in a patient care and management. Emergence of colistin resistant strains of K. pneumoniae is alarming. Therefore, early detection and prompt implementation of infection control measures is important to prevent further spread of MDR K. pneumoniae.

Keywords

Klebsiella pneumoniae, MDRO, colistin resistant, broth micro dilution