Rapid Diagnosis of Pulmonary and Extrapulmonary Tuberculosis by Cartridge-Based Nucleic Acid Amplification Test (CBNAAT)

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Original Article

Author Details : Saurabh Jain, Ramesh Agrawal*

Volume : 5, Issue : 4, Year : 2019

Article Page : 204-206

https://doi.org/10.18231/j.ijmmtd.2019.046

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Abstract

Introduction: Tuberculosis incidence in India is 2.74 million including 0.13 million drug resistant cases.
According to WHO 0.41 million death in India due to tuberculosis in 2017. Sputum smear microscopy is
facing variable sensitivity issue particularly in patients with sputum smear-negative and/or extrapulmonary
disease, and drug-resistant Tuberculosis. To overcome this problem, WHO’s current policies and guidance
recommend to use Xpert MTB/RIF as an initial diagnostic test.
Materials and Methods: This study done at RNTCP lab, Microbiology Dept., Govt. Medical College
Khandwa, M.P., India, is a Retrospective observational record based analysis from 01 January 2018 to 31
May 2019 (1 year 5 months) on 1993 samples.
Results: Out of 1736 samples, 1537 were pulmonary & 199 Extrapulmonary included in this study.
Samples positivity rate were (727) 41.87 %, of which (680) 39.17% Rifampicin sensitive & (47) 2.7%
resistant for Mycobacterium tuberculosis. Pleural fluid 43.7%, Pus 27% & cervical lymph node 16.6%
were most common extrapulmonary sample. 37 extrapulmonary samples were positive for Mycobacterium
tuberculosis, out of this 33 sensitive & 4 were resistant to Rifampicin.
Discussion: Rifampicin resistance by CBNAAT was found to be 6.47% in our study. Gour Sanjay M et al
found this 6.38%, 13.55% by D. Pragati Rao et al, 25% by R Dewan et al & very high (53%) by R Tripathi
et al in their study. Pleural fluid (43.7%) was the most common extrapulmonary samples followed by Pus
(27%) & lymph node (16.6%). Gour Sanjay M et al also found Pleural fluid 49.81% as most common
sample followed by lymph node. 40%, Pus 37.73%. Extrapulmonary sample distribution pattern like our
study.
Conclusion: Rifampicin resistant Mycobacterium tuberculosis is low in our area as compare to other part
of India.

Keywords: MTB, CBNAAT.

How to cite : Jain S, Agrawal R, Rapid Diagnosis of Pulmonary and Extrapulmonary Tuberculosis by Cartridge-Based Nucleic Acid Amplification Test (CBNAAT). IP Int J Med Microbiol Trop Dis 2019;5(4):204-206

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