Detection of biofilm formation, extended spectrum beta lactamase production and their correlation with antibiotic resistance among uropathogenic Escherichia coli

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Original Article

Author Details : D E Premlatha, Vijetha Sajjanar*

Volume : 7, Issue : 3, Year : 2021

Article Page : 141-147

https://doi.org/10.18231/j.ijmmtd.2021.031

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Abstract

Background: Urinary tract infections (UTI) are most common bacterial infections encountered in clinical practice and E.coli is predominant organism causing UTI.
Objectives of the study: 1. To study antibiotic resistance pattern among uropathogenic E.coli (UPEC). 2. To study extended spectrum beta lactamase (ESBL) production and their correlation with antibiotic resistance among uropathogenic E.coli (UPEC). 3. To study biofilm formation and its correlation with antibiotic resistance among uropathogenic E.coli (UPEC).
Materials and Methods: A prospective study was conducted on first 100 Escherichia coli isolated from urine specimens of patients suspected to be having urinary tract infection between January 2016 and December 2016 received at Department of Microbiology, SIMS Shimoga. Fresh midstream urine samples were aseptically collected in sterile containers and plated on Blood agar & MacConkey agar plates using a standard loop technique & the growth was processed by standard bacteriological technique. Biofilm detection was done by tube and microtitre plate method. ESBL detection was done according to CLSI criteria. Antimicrobial sensitivity testing was done using Kirby-Bauer methods on Mueller-Hinton agar. Results were interpreted as per the CLSI guidelines.
Result: Antibiotic sensitivity of E.coli was nitrofurantoin (100%) fosfomycin (100%) imipenem (77%) cotrimoxazole (61%) amikacin (47%) aztreonam (53%) piperacillintazobactam (41%). cefotaxim (25%) ceftazidime (32%) norfloxacin (20%) ampicillin-sulbactam (12%) ciprofloxacin (15%), levofloxacin (12%), amoxiclavulanicacid (15%). ESBL positive were 83% (screening), 80% (confirmatory). Biofilm positive were 63% (tube method) 79% (Microtiterplate method).
Conclusion: This study showed Fosfomycin and Nitrofurantoin were the most sensitive drug. Study shows antibiotic resistance was seen more in ESBL and Biofilm producers compared to non ESBL and non biofilm producers.

Keywords: Antibiotic resistance, ESBL, Biofilm, UTIUPEC

How to cite : Premlatha D E, Sajjanar V, Detection of biofilm formation, extended spectrum beta lactamase production and their correlation with antibiotic resistance among uropathogenic Escherichia coli. IP Int J Med Microbiol Trop Dis 2021;7(3):141-147

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