Antimicrobial activity of Lactobacillus acidophilus bacteriocin against clinical isolates

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Original Article

Author Details : Wedad Mohammed Al-Haik, Yasser Mansour Matran, Ibrahim Mohammed Bawazir, Ahmed Mohammed Al-Haddad*

Volume : 9, Issue : 3, Year : 2023

Article Page : 182-188

https://doi.org/10.18231/j.ijmmtd.2023.035

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Abstract

Background: Lactic acid bacteria serve as important human probiotics, as they can produce bacteriocins from different dairy sources, and the antimicrobial activity of their bacteriocins have been documented in the scientific field. This study aimed to assess the antimicrobial activities of Lactobacillus acidophilus bacteriocin obtained from different dairy sources.
Materials and Methods: An experimental analytical design was used to isolate Lactobacillus species on de Man, Rogosa and Sharpe Media, and identify them phenotypically and evaluated their bacteriocin’s antimicrobial activity against four clinical isolates. SPSS Version 22 was used for Statistical purposes. 
Results: A total of 14 Lactobacillus isolates were verified from various sample sources. These included 6 isolates from camel’s milk, 2 from goat’s milk, 3 from local yogurt, and 3 from Omani yogurt. The supernatants of most Lactobacillus isolates exhibited varying levels of antimicrobial activity during the investigation. Furthermore, the action of the bacteriocin was concentration dependent. The highest level of inhibition was 14 mm for Staphylococcus aureus, 12 mm for Bacillus subtilis, 12 mm for Escherichia coli, and 10 mm for Salmonella paratyphi when using 100 l of raw bacteriocin. Moreover, the correlation between bacteriocin activity, temperatures, and incubation times was found to be irreversible.
Conclusions: The study reveals inherent antibacterial traits in Lactobacillus species from local milk and milk products in Al-Shihr Town. Specifically, L. acidophilus variant, synthesizing bacteriocin, effectively suppressed the clinical isolates, showcasing their pathogen-fighting ability. These findings offer potential for developing efficient antimicrobial methods, utilizing L. acidophilus bacteriocin as an antibiotic in food and medicine.
 
Keywords: Bacteriocin, Lactobacillus, Antimicrobial Activity, L acidophilus

How to cite : Al-haik W M, Matran Y M, Bawazir I M, Al-haddad A M, Antimicrobial activity of Lactobacillus acidophilus bacteriocin against clinical isolates. IP Int J Med Microbiol Trop Dis 2023;9(3):182-188

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