Study of bacteriological profile and antibiotic susceptibility pattern of pus isolates in tertiary care hospital

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Original Article

Author Details : Vijetha Sajjanar*, Premalatha DE, Siddesh KC, Prakash N

Volume : 9, Issue : 4, Year : 2023

Article Page : 253-257

https://doi.org/10.18231/j.ijmmtd.2023.048

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Abstract

Background: Surgical wound causes invasion of pathogens causing surgical site infections which are commonly polymicrobial in nature. This pus forming infection causes delayed wound healing, wound dehiscence and wound breakdown contributing to important healthcare associated infections (HAI) Multidrug resistance has emerged among organisms isolated in pus sample due to failure of appropriate use of antibiotics.
Objectives of the study: 1. To study the bacteriological profile of pus samples 2. To determine the antibiotic susceptibility pattern of isolated pathogens from pus samples.
Materials and Methods: The study was conducted in department of microbiology, Shimoga institute of medical sciences, Shimoga from January 2018 to June 2018.All pus samples were processed on blood agar, MacConkey agar and incubated at 37?c under aerobic conditions for 24 hours. The organisms were identified as per standard conventional methods. The antimicrobial susceptibility tests were done by Kirby–Bauer’s Disk Diffusion method on Mueller–Hinton Agar and interpreted as per clinical laboratory standard institution guidelines (CLSI).
Result: Out of 350 samples 250 were culture positive 100 were culture negative. Among culture positive most common organism isolated was followed by Staphylococcus aureus 86(34.45%), Klebsiella spp 72(28.8%), Pseudomonas Aeruginosa 55(22%), E. coli 20(8%), CoNS 9(3.6%), Proteus spp 2(0.8%), Enterococcus spp 3(1.2%) Acinetobacter spp 3(1.2%), Citrobacter spp 3(1.2%). Gram positive organisms were most sensitive for linezolid, vancomycin and least sensitive to cefoxitin, erythromycin. Gram Negative Organisms Were Most Sensitive for Imipenam, Piperacillin tazobactam and least Sensitive for Ampicillinsulbactam,
Ciprofloxacin.
Conclusion: Staphylococcus aures is most common etiology of pus forming infection most importantly surgical site infections (SSI). MRSA prevalence in hospital set up indicates the failure of proper infection control practices implementation in the hospitals causing healthcare associated infections (HAI). Emergence of multidrug resistance among the pus isolates is because of non-judicious use of antibiotics.
 
Keywords: Antibiotic susceptibility, PUS, Bacteriological profile, MRSA, Hospital acquired infection (HAI)

How to cite : Sajjanar V, Premalatha De, Siddesh Kc, Prakash N, Study of bacteriological profile and antibiotic susceptibility pattern of pus isolates in tertiary care hospital. IP Int J Med Microbiol Trop Dis 2023;9(4):253-257

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