Assessment of transaminase enzymes and effect of potassium iodide on its production in the yeast form of Sporothrix schenckii

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Original Article

Author Details : Sumedha , Shinu Pottathil, Rajesh Bareja*

Volume : 10, Issue : 2, Year : 2024

Article Page : 101-107

https://doi.org/10.18231/j.ijmmtd.2024.019

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Abstract

Background: The dimorphic fungus Sporothrix schenckii (S. schenckii) is the cause of sporotrichosis. Many fungi have transaminases, also known as aminotransferases, which are portions of proteins. However, nothing is known about this enzyme in S. schenckii.
Aims: The current study shows how potassium iodide (KI) affects the transaminases enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) produced by S. schenckii’s yeast form in vitro. 
Materials and Methods: YNB (yeast nitrogen base) medium was used to create a master culture of S. schenckii, which was then incubated at 37ºC (yeast). The amount of KI added to the YNB medium increased. Each container received one millilitre (mL) of the master culture suspension, which was then incubated at 37ºC for different lengths of time – the early-log phase on day six, the mid-log period on day twelve, and the growth peak on day eighteen, respectively. A 5% homogenate was produced after
centrifugation and used in the transaminases enzyme assay.
Results: On days 6, 12, and 18, the control specimen’s mean aspartate aminotransferase level was 10.11  3.09, 10.36 2.33, and 17.62 4.27 IU, respectively. On days 6, 12, and 18, the test specimen’s mean aspartate aminotransferase level ranged from 9.80 2.42 (KI 0.4 gramme %) to 19.59 3.9 IU (KI 0.2 gramme %), from 4.52 2.28 (KI 0.4 gramme %) to 28.46 4.88 IU (KI 0.2 gramme %), and from 4.50  1.02 (KI 0.8 gramme %) to 14.49 3.60 IU (KI 0.05 gramme %). On days 6, 12, and 18, the control specimen’s mean alanine aminotransferase level was 10.70 3.82, 29.60 3.02, and 19.74 4.62 IU, respectively. Day 6, 12, and 18 saw variations in the test specimen’s mean alanine aminotransferase level, which ranged from 11.40 3.04 (KI 0.1 gramme %) to 18.52 3.97 IU (KI 0.2 gramme %), 7.82 1.50 (KI 0.8 gramme %) to 41.56 4.56 IU (KI 0.2 gramme %), and 3.33 0.70 (KI 0.8 gramme %) to 12.54  1.92 IU.
Conclusions: The transaminase enzymes’ low activity indicates that KI has an inhibiting effect on the growth of S. schenckii (yeast), which has caused a drop in the enzymes’ activity. 
The impact of KI on the lipids of S. schenckii may be monitored to comprehend the mechanism of action of KI in the future..

Keywords: Aspartate aminotransferase, Alanine aminotransferase, Yeast, KI

How to cite : Sumedha, Pottathil S, Bareja R, Assessment of transaminase enzymes and effect of potassium iodide on its production in the yeast form of Sporothrix schenckii. IP Int J Med Microbiol Trop Dis 2024;10(2):101-107

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