Phenotypic detection of ESBL and Amp C beta lactamase among clinical isolates of Enterobacteriaceae

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Original Article

Author Details : Vrushali Thakar*, Brundha Gandavalli, Deepa Devhare, Meera Modak, Shailaja Sawant, Mahadevan Kumar, Sunita Bhatawadekar

Volume : 10, Issue : 2, Year : 2024

Article Page : 130-137

https://doi.org/10.18231/j.ijmmtd.2024.024

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Abstract

Introduction: Emergence of ESBL & amp C -Lactamase enzymes among clinical isolates of Enterobacteriaceae poses a significant public health concern. Antimicrobial agents like 3rd generation cephalosporins, clavulanic acid, imipenem etc. can induce amp C enzyme production. Sometimes the automated antimicrobial susceptibility testing systems fails to detect Amp C phenotype due to presence of multiple resistance mechanisms in gram negative bacilli. To overcome such difficulty, the study was
undertaken to detect the presence ESBL and Amp C enzymes among clinical isolates of Enterobacteriaceae by phenotypic methods.
Materials and Methods: Enterobacteriaceae strains isolated from various clinical samples were included in the study. Strains resistant to two or three groups of antibiotics (MDR) were further tested for the presence of ESBL enzyme by combination disk method. Amp C enzymes were detected by cefoxitincloxacillin disc method.
Results: A total 1059 Enterobacteriaceae strains were isolated from various clinical samples. Out of these, 170 MDR strains were further processed. ESBL enzymes were detected in 104 (61%) strains and Amp C in 35 (20.5%) strains. 26(15.2%) strains were co-producers.
Conclusion: Detection of ESBL and Amp C enzymes will help clinician in choosing the right antimicrobial treatment for the patient. Routine reporting of presence of Amp C & ESBL enzymes by phenotypic methods can be easily implemented by clinical microbiology laboratory. Cefoxitin-cloxacillin disk test is simple & rapid method for detection of Amp C - lactamase enzyme.
 

Keywords: Enterobacteriaceae, Amp C beta lactamase, ESBL

How to cite : Thakar V, Gandavalli B, Devhare D, Modak M, Sawant S, Kumar M, Bhatawadekar S, Phenotypic detection of ESBL and Amp C beta lactamase among clinical isolates of Enterobacteriaceae. IP Int J Med Microbiol Trop Dis 2024;10(2):130-137

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