Prevalence, antimicrobial resistance patterns and ESBL resistance genotypes of Klebsiella pneumoniae Isolated from patients presenting with urinary tract infections at Mama Lucy Hospital, Kenya

---

Original Article

Author Details : Liliane Muhimpundu*, Sepha Mabeya, John Njeru Mwaniki, Gabriel Miringu, Caroline Ngugi

Volume : 10, Issue : 4, Year : 2024

Article Page : 416-427

https://doi.org/10.18231/j.ijmmtd.2024.068

Suggest article by email

Get Permission

---

Abstract

Background: Multidrug -resistance in Klebsiella pneumoniae has increased rapidly and present a global public health challenge. This challenge has resulted from the Extended Spectrum Beta-Lactamase (ESBL) producers rendering most of beta-lactam antibiotics ineffective posing a therapeutic threat. This study was carried out to determine the prevalence, antimicrobial susceptibility profiles, and ESBL specific resistant genes in K. Pneumoniae isolated from urinary tract-infected patients at Mama Lucy Hospital. 
Materials and Methods: A cross-sectional study was conducted among consenting 400 suspected urinary tract infected patients visiting Mama Lucy hospital, Kenya. Midstream urine samples were collected, cultured,identified and antimicrobial susceptibility patterns determined using the disc diffusion method and ESBL production detected using double disc synergy test. The ESBL positive phenotypes were then confirmed by PCR targeting four beta-lactamase blaSHV, blaTEM, blaCTX-M and blaOXA genes.
Results: A total of 400 participants were found to have UTIs. The females’ participants were 77.25% while males were 22.75%. The majority participants were between 30-35 years of age (33.5%). Out of the total 400 participants, K. pneumoniae accounted for 40 (19.41%) out of which 30 (75%) were confirmed ESBL positive. High resistance was observed against Ampicillin (84.37%), while 40.62% showed an equal resistance to Ceftriaxone, and Cefotaxime and Sulfamethoxazole-trimethoprim with
37.50%. Chloramphenicol 96.87%, and Nitrofurantoin 90.62% were effective to isolates. None of the isolates showed resistance to Imipenem. The blaTEM (66.66%) was the most prevalent ESBL gene detected, blaSHV at 60%, blaCTX-M at 40% and the least was blaOXA at 3.33%.
Conclusion: The high prevalence of Klebsiella pneumoniae, and high trends of ESBL resistance genes were observed. The routine laboratory testing for ESBL phenotypic and molecular methods is recommeded to optimize antibiotic management and reduce the risk of spread of infections caused by ESBL producers. 

Keywords: Klebsiella pneumoniae, Antimicrobial, ESBL genes

How to cite : Muhimpundu L, Mabeya S, Mwaniki J N, Miringu G, Ngugi C, Prevalence, antimicrobial resistance patterns and ESBL resistance genotypes of Klebsiella pneumoniae Isolated from patients presenting with urinary tract infections at Mama Lucy Hospital, Kenya. IP Int J Med Microbiol Trop Dis 2024;10(4):416-427

This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.