A study on the prevalence of vancomycin resistant and intermediate staphylococcus aureus isolated from various clinical Specimen in a tertiary care hospital and detection of their MIC values by E-test

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Original Article

Author Details : Anjali Kulshrestha, V. Anamika, K. Mrithunjay, A.S. Dalal, K. Manish

Volume : 3, Issue : 3, Year : 2017

Article Page : 119-125

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Abstract

The main antimicrobial used to treat MRSA infections which are life threatening is a glycopeptide antibiotic, Vancomycin. Inturn extensive use of vancomycin in various parts of the world lead to the a rise in Staphylococcus aureus strains which are resistant to vancomycin.^(1,2) Hence this study was carried out to detect vancomycin resistance in S.aureus isolated from various clinical samples. All 161 Staphylococcus aureus strains were screened for Vancomycin resistance by BHI - Vancomycin screen agar [6µg/ml] and Vancomycin disc diffusion method [30µg/disc]. These were further confirmed by E- test. 82 (51%) strains were MRSA determined by Cefoxitin disc diffusion method and MRSA were maximally isolated from pus (61%). 45% strains were MDR-MRSA. Only 5 S.aureus strains showed resistance to Vancomycin by disc diffusion method, but all these strains were sensitive to Vancomycin by both Vancomycin screen agar [6µg/ml] and E- test, thus this study also showed 100% sensitivity and specificity for BHI –VSA (6µgm/ml). As current study only indicates the tip of iceberg. Hence we suggest, more and more studies should be undertaken in future to monitor the emergence of resistance to these antibiotic.

Keywords: VSSA, VRSA, MRSA, Multidrug resistance.

How to cite : Kulshrestha A, Anamika V, Mrithunjay K, Dalal A, Manish K, A study on the prevalence of vancomycin resistant and intermediate staphylococcus aureus isolated from various clinical Specimen in a tertiary care hospital and detection of their MIC values by E-test. IP Int J Med Microbiol Trop Dis 2017;3(3):119-125

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