Detection of Siderophore production in Uropathogenic Escherichia.coli in patients with Type 2 Diabetes Mellitus

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Short Communication

Author Details : Mehvish Saleem, Betty Daniel

Volume : 3, Issue : 4, Year : 2017

Article Page : 176-177

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Abstract

Introduction: Infection of the urinary tract is a common observation in patients with type 2 diabetes mellitus. UTI can be a severe complication in diabetic patients. The aim of this study was to determine siderophore production as a virulence factor in Escherichia.coli, commonly responsible for UTI.
Material and Methods: The study was conducted at the postgraduate department of Botany (Microbiology Unit) of St. Josephs College PG & research center Bangalore India between January 2010 - February 2011. Midstream urine from diabetic patients was collected and E.coli was isolated and identified using standard microbiology protocols Growth was examined after 24 hours of incubation at 37⁰c. Colonies more than 10⁵cfu/ml were considered significant for UTI. Detection of siderophores was performed using Chrome Azurol Assay (CAS)
Results: Siderophore production was observed in 190(95%) of E.coli isolates and one of the 20 (5%) among controls.
Conclusion: Iron acquisition or siderophores are a major virulence factor necessary for Uropathogenic E.coli in the pathogenesis of UTI in diabetic patients. CAS assay is the most efficient method for the detection of siderophores.

Keywords: Diabetes Mellitus, UPEC, UTI Siderophores.

 

How to cite : Saleem M, Daniel B, Detection of Siderophore production in Uropathogenic Escherichia.coli in patients with Type 2 Diabetes Mellitus. IP Int J Med Microbiol Trop Dis 2017;3(4):176-177

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