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- DOI 10.18231/j.ijmmtd.2019.036
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CrossMark
- Citation
Antibiogram of Pseudomonas aeruginosa Isolated from Infected wounds
- Author Details:
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Manmeet Kaur Gill *
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Ashish Khanna
Introduction: The wounds infection remains a medical problem worldwide due to associated morbidity, mortality and economic burden. Wounds can be infected with bacteria or yeast and the source is usually hosts normal flora and/or the hospital environment. Since Pseudomonas aeroginosa is the most common gram-negative non fermenter opportunistic bacteria associated with nosocomial infections. The present study was undertaken to know the antibiotic susceptibility patterns of this pathogen.
Materials and Methods: It was a cross-sectional study carried out over a period of one year. Pus samples collected at source were processed as per standard bacteriological techniques for aerobic cultures. They were inoculated on 5% Blood agar and Mac-Conkey’s agar and incubated aerobically for 24-48 hours at 37 degree before reporting them as sterile. Anaerobic cultures were not done. Non lactose fermenting colonies on MacConkey agar were subjected to oxidase test. These oxidase positive isolates were further identified by Vitek-2 Compact (Biomerieux) using gram negative identification cards, following manufacturer’s guidelines. Antimicrobial sensitivity was also determined by same system using 281 AST cards. Antibiotic sensitivity results were interpreted as per CLSI guidelines.
Results: A total of 1566 wound swab/pus samples from patients with infected wounds were processed for culture and sensitivity testing from January 2018 to November 2018.
The present study comprised of 101 isolates of Pseudomonas aeroginosa. Isolation rate was highest in the age group 20-40 years followed by those above 60 years of age. Most of the isolates obtained were multi-drug resistant. Highest sensitivity was seen in Amikacin and Imipenem.
Conclusion: In conclusion, Pseudomonas aeroginosa which is a common Gram negative bacteria isolated from wound infections and also highly resistant to different types of antibiotics could be a reason for high morbidity and mortality in hospitalised patients. Therefore a regular surveillance of antibiotic resistance of these organisms is needed to prevent indiscriminate use of antibiotics leading to emergence of drug resistance among these pathogens.
Keywords: Pseudomonas aeroginosa, Nosocomial infections, Multi-drug resistant.
References
- Kumburu HH, Sonda T, Mmbaga BT, Alifrangis M, Lund O, Kibiki G, et al. Patterns of infections, aetiological agents and antimicrobial resistance at a tertiary care hospital in northern Tanzania. Trop Med Int Health 2017;22:454–64.
- Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, et al. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen 2009;17:763–71.
- Chen, Y, Zhou Z, Jiang Y. Emergence of NDM-1-producing Acinetobacter baumannii in China.". J Antimicrob Chemother 2011;66:1255–9.
- Gill MK, Sharma S. Bacteriological profile and antibiotic sensitivity patterns of aerobic pus isolates: A study conducted in tertiary care hospital of North India. Int J Med Microbiol Trop Dis 2019;5(2):99-102.
- Hujer K.M., Hujer A.M., Hulten E., Bajaksouzian S., Adams J.M., Donskey C.J et al. Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter sp. isolates from military and civilian patients treated at the Walter Reed Army Medical Center. Antimicrob. Agents and Chemother 2006;50: 4114–23.
- Cheesbrough M: District laboratory practice in tropical countries volume II: microbiology. Cambridge (UK): Cambridge University Press; 2006:1–479.
- Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing. Twent Inf 2018;28th ed:1–249.
- F. Gottrup, A. Melling, and D. A. Hollander, “An overview of surgical site infections: aetiology, incidence and risk factors,” World Wide Wounds 2005;5(2):11–15.
- R. M. Mordi and M. I. Momoh, “Incidence of Proteus species in wound infections and their sensitivity pattern in the University of Benin Teaching Hospital,” Afr J Biotechnol 2009;8(5):725–30.
- Hancock RE. Resistance mechanisms in Pseudomonas aeruginosa and other nonfermentative gram-negative bacteria. Clin Infect Dis 1998;27(1):S93–S99. doi: 10.1086/514909
[Google Scholar] - Parajuli NP, Acharya SP, Mishra SK, Parajuli K, Rijal BP, Pokhrel BM. High burden of antimicrobial resistance among gram negative bacteria causing healthcare associated infections in a critical care unit of Nepal. Antimicrob Resist Infect Control. 2017;6:67. Published 2017 Jun 15. doi:10.1186/s13756-017-0222-z
[Google Scholar] - AL Kassi, A., AL Thwani, A., AL Segar, R. PCR detection of some ESBLS genes in Pseudomonas aeruginosa isolated from Burn’s units in Baghdad hospital Baghdad". J Biotechnol Res Center 2015;9(2):74.
- L-Kadhmi, N.A., A.N. AL-Thwaini, W.A. AL-Turk and ALtaif, K.I. Studies on the Multidrug Resistance to Pseudomonas aeruginosa Isolated from Infected Wounds. Int J Curr.Microbiol App Sci 2016;5(5):963-970. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.101
[Google Scholar]
How to Cite This Article
Vancouver
Gill MK, Khanna A. Antibiogram of Pseudomonas aeruginosa Isolated from Infected wounds [Internet]. IP Int J Med Microbiol Trop Dis. 2019 [cited 2025 Oct 19];5(3):163-165. Available from: https://doi.org/10.18231/j.ijmmtd.2019.036
APA
Gill, M. K., Khanna, A. (2019). Antibiogram of Pseudomonas aeruginosa Isolated from Infected wounds. IP Int J Med Microbiol Trop Dis, 5(3), 163-165. https://doi.org/10.18231/j.ijmmtd.2019.036
MLA
Gill, Manmeet Kaur, Khanna, Ashish. "Antibiogram of Pseudomonas aeruginosa Isolated from Infected wounds." IP Int J Med Microbiol Trop Dis, vol. 5, no. 3, 2019, pp. 163-165. https://doi.org/10.18231/j.ijmmtd.2019.036
Chicago
Gill, M. K., Khanna, A.. "Antibiogram of Pseudomonas aeruginosa Isolated from Infected wounds." IP Int J Med Microbiol Trop Dis 5, no. 3 (2019): 163-165. https://doi.org/10.18231/j.ijmmtd.2019.036