Invitro studies on colon cancer cell inhibition by cell free extracts of natural probiotics and Bacillus clausii

• Author Details:   
• Pranav Suresh Tambe
• Ranjana Shirish Chavan
• Shilpa Pranav Tambe
• Gajanan Vishnu Mali ^*

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Background: Colorectal cancer (CRC) is an increasingly concerning health issue, which signifies in searching for new alternatives to minimize the side effects associated with chemotherapy and radiation therapy. Probiotic organisms belonging to genus Lactobacilllus and other genera and their derivatives are gaining attention now a days for their potential role in this area.
Materials and Methods:  In this study, cell-free extracts from four probiotic isolates-LC-8, LC-20, LC-27, LC-30 and Bacillus clausii whose sporulating suspension for maintaining gut microbiota were tested against the HCT-116 and COLO-205 cell lines. MTT assay was done to determine IC-50 values. Wound healing and colony formation assays at half and double IC-50 values were done in order to study cell signalling and colony formation in time and dose dependent manner.
Results:  Two isolates LC-20 and LC-30 showed promising activities against HCT-116 cells as compared to COLO-205 cells. These crude cell free extracts showed significant antiproliferative effects when compared to the standard drug Adriamycin (ADR). Further investigation to inhibit the cell signalling and wound healing activities of cancer cells with the help of these extracts, tested at half and double IC-50 values, showed that the extracts effectively inhibited cell signalling and migration.
Conclusion: The findings suggest that cell-free extracts from probiotic organisms possess potent antiproliferative properties, making these postbiotics as a promising alternative or combination with chemotherapy for CRC treatment.

Keywords: CRC, HCT-116, COLO-205, Cell migration, Clonogenic assay, Chemotherapy