Agreement between AST from minute colony (8-10h growth) and mature colony (16-18h/overnight incubation)

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Original Article

Author Details : Sonali Padhy, Ketan Priyadarshi, Sarumathi Dhandapani, Stessy Ann Punnen, Apurba S Sastry*

Volume : 10, Issue : 3, Year : 2024

Article Page : 219-225

https://doi.org/10.18231/j.ijmmtd.2024.038

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Abstract

Background: Sepsis is a medical emergency where a successful patient outcome depends on early and appropriate antibiotic treatment. This study was conducted to evaluate agreement between antimicrobial susceptibility testing (AST) from minute colonies that can reduce the AST-TAT by as much as 8-10h as compared to the CLSI recommended protocol of performing AST from an overnight (16-18h) growth of mature colony.
Materials and Methods: In the present study, mDD results from minute colony (8-10h growth) were compared to the rDD results mature colony (16-18h/overnight incubation) CA and various types of errors were evaluated.
Results: 237 pathogens and 1597 organism-antibiotic combinations were evaluated, there was a CA of 93.30% which was extremely satisfactory and categorical disagreement was found only in 4.56% of organism-antibiotic combinations, which were mainly mE (4.56%) with nil VME (0%) and ME (0%).
Conclusion: We have found that minute colony (8-10h) AST is in agreement with reference mature colony (16-18h) AST, shortening TAT by (8-10h) earlier than the conventional reference method which is very helpful in treatment of sepsis patients.
 

Keywords: Sepsis, AST­TAT, minute colony, mature colony

How to cite : Padhy S, Priyadarshi K, Dhandapani S, Punnen S A, Sastry A S, Agreement between AST from minute colony (8-10h growth) and mature colony (16-18h/overnight incubation). IP Int J Med Microbiol Trop Dis 2024;10(3):219-225

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