Detection of antibiotic resistance and metallo beta lactamase among Pseudomonas aeruginosa clinical isolates

Authors: Dr.Vijayashree MBBS MD, Ganesh Perumal PerumalSamy, Dr.Banumathy MBBS MD, Dr.A.R.Preetthi MBBS MD

DOI: 10.18231/j.ijmmtd.12225.1761651771

Keywords: Key words: Modified Hodge, MBL,E-test.

Abstract: Introduction: MBL generating Pseudomonas aeruginosa (MBL-PA) have a significant clinical problem that poses a significant obstacle to antimicrobial therapy. Aim: The aim of this study was to detect the metallo-β-lactamase(MBL) production in meropenem(MRP) resistant P.aeruginosa isolates. Materials and methods: The research was carried out between August 2013 and July 2014. The study includes strains of Pseudomonas aeruginosa that were obtained from a variety of clinical specimens at the Coimbatore Medical College's Department of Microbiology. There were around 211 isolates found. In order to demonstrate which phenotypic test was the most dependable for the prompt identification of MBL from meropenem-resistant isolates, the study aimed to ascertain the prevalence of MBL from pseudomonad isolates and compare the Modified Hodge Test (MHT), Combined disc test (CDT), and Double Disc synergy test (DDST) with the E-test. Results: The prevalence of MBL in P.aeruginosa isolates was 7.11%. The majority of meropenem resistant isolates produces MBL. Of the four phenotypic tests, the E test alone had the highest sensitivity(100%) and specificity(100%) as well as the highest positive predictive value(100%) among all four tests. Conclusions: The E-test was the most effective and trustworthy phenotypic test that allowed for detection of the isolate's MIC(minimum inhibitory concentration) value. The next more dependable tests for detecting MBL after the E-test were CDT and DDST, which were followed by MHT.