Molecular Characterization of Pseudomonas aeruginosa Isolates from Chronic Kidney Disease Patients

Authors: Haider Sabah Abdulhussein, Sarah A. Al-khafaji, Moslim Mohsin Khalaf

DOI: 10.18231/j.ijmmtd.13326.1761984669

Keywords: Pseudomonas aeruginosa, chronic kidney disease, oprL gene, toxA gene, exoS gene, molecular characterization, virulence factors.

Abstract: Pseudomonas aeruginosa is an important opportunistic pathogen that causes sufficient risk of chronic kidney disease (CKD) in patients due to their compromised condition. P. aeruginosa The molecular isolates are important for understanding the wireless pattern and improving therapeutic results in this weak population. Objective: The purpose of this study is molecularly P. aeruginosa is isolated from CKD patients using clinical genes OPRL and PCR-based detections of OPRL and wireless genes and XOS. Methods: A total of 85 P. aeruginosa from various clinical samples of CKD patients. Bacterial identity was confirmed by the use of traditional methods and PCR amplification of the OPRL gene. The viral gender detection was performed using multiplex PCR targeting of toxa and exos genes. CLSI was made according to the guidelines tested for antimicrobial sensitivity. Results: All 85 isolates (100%) were positive for the oprL gene, confirming their identity as P. aeruginosa. The prevalence of virulence genes was toxA 78.8% (67/85) and exoS 64.7% (55/85). Co-occurrence of both toxA and exoS genes was observed in 52.9% (45/85) of isolates. Isolates harboring both virulence genes demonstrated significantly higher antibiotic resistance rates compared to those with single or no virulence genes (p < 0.05). Conclusion: P. aeruginosa isolates from CKD patients exhibit a high prevalence of virulence genes toxA and exoS, with a significant correlation between virulence gene presence and antibiotic resistance. Molecular characterization provides valuable insights for targeted therapeutic approaches in this high-risk patient population.