Evaluation of multidrug resistant pathogens in wound infections: Bacteriological profiles and clinical outcome implication

• Author Details:   
• Srilalitha Vishnubhatla ^*
• Satyanarayana Oruganti
• Padmaja N

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Background: One of the most common presentations in the outpatient department is pyogenic or wound infections. Multidrug-Resistant (MDR) bacterial infection is considered as one of the significant risk factor for impaired wound healing. The aim of this study is analysing bacteriological profiles and antibiotic sensitivity patterns from pus samples, and examine how these organisms correlate with patient outcomes.
Materials and Methods: During the period of retrospective cross-sectional study, around 940 pus samples from wounded sites were collected and processed in Microbiology department as per the standard guidelines. Clinical data was also analysed to correlate MDRO presence with infection outcomes. All prepared biochemical and streaking media were checked for their sterility. Quality control Strains were used as reference strains for quality control of AST and biochemical tests.
Results: Majority of the organisms isolated were Pseudomonas (23.0%), Staphylococcus aureus (21%), E.coli (17.5%), Klebsiella (17.2%). ESBL production was observed more in E.coli (58.4%) followed by Klebsiella (50.9%) and Proteus (24.2%). MBL production was noted in 27.3% isolates of E.coli, 21.1% of Klebsiella and 6.06% of Proteus isolates. ESBL production was observed more in Pseudomonas (52.1%) followed by Acinetobacter (51.6%). MBL production was noted in 32.2% isolates of Pseudomonas, and 41.7% of Acinetobacter isolates. MRSA was noted in 77.08% isolates of wound infections.
Conclusion: Our study highlights that MDRO-infected wound require prolonged hospital stays, intensive interventions, and alternative therapies due to limited antibiotic options. Effective antimicrobial stewardship, rapid diagnostics, and strict infection control measures are essential to curb resistance and improve outcomes.

Keywords: Multidrug resistant pathogens, Wound infections, Microbiotia