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- DOI 10.18231/j.ijmmtd.v.11.i.3.2
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In-vitro antimicrobial activity of Oscimum sanctum leaf extract against pathogens isolated from otitis media infection
Introduction: Otitis media (OM) is the primary contributor to hearing loss in children younger than 5 years, a vital time for the development of speech, language, and cognition. Medications obtained from plants are favoured over synthetic treatments because of their potent pharmacological effects, low toxicity, and cost-effectiveness. O. sanctum has traditionally been used to treat various infections.
Aims and Objective: This study highlights the need for molecular identification of microorganisms linked to OM infection and examining the antimicrobial effects of extracted leaves from O. sanctum on OM infection pathogens.
Materials and Methods: The OM swab samples were processed for microbial identification and antimicrobial susceptibility through the Vitek 2 system, and molecular identification was carried out to identify the pathogens. The five non-polar to polar solvents were used to evaluate the zone of inhibition of all solvents against S. aureus, P. aeruginosa, A. baumannii, K. pneumoniae, and C. albicans. The highest ZOI was reported with methanolic extract against S. aureus, and the best MIC of 0.162 mg/ml was found against C. albicans.
Conclusion: In this study, the O. sanctum methanolic leaves extract has shown good inhibitory activity against OM drug-resistant pathogens.
How to Cite This Article
Vancouver
Dash J, Das SR, Purohit GK, Sahu KK, Tripathy SK, Dubey D. In-vitro antimicrobial activity of Oscimum sanctum leaf extract against pathogens isolated from otitis media infection [Internet]. IP Int J Med Microbiol Trop Dis. 2025 [cited 2025 Sep 06];11(3):250-256. Available from: https://doi.org/10.18231/j.ijmmtd.v.11.i.3.2
APA
Dash, J., Das, S. R., Purohit, G. K., Sahu, K. K., Tripathy, S. K., Dubey, D. (2025). In-vitro antimicrobial activity of Oscimum sanctum leaf extract against pathogens isolated from otitis media infection. IP Int J Med Microbiol Trop Dis, 11(3), 250-256. https://doi.org/10.18231/j.ijmmtd.v.11.i.3.2
MLA
Dash, Jayanti, Das, Saumya Ranjan, Purohit, Gopal Krishna, Sahu, Kundan Kumar, Tripathy, Subrat Kumar, Dubey, Debasmita. "In-vitro antimicrobial activity of Oscimum sanctum leaf extract against pathogens isolated from otitis media infection." IP Int J Med Microbiol Trop Dis, vol. 11, no. 3, 2025, pp. 250-256. https://doi.org/10.18231/j.ijmmtd.v.11.i.3.2
Chicago
Dash, J., Das, S. R., Purohit, G. K., Sahu, K. K., Tripathy, S. K., Dubey, D.. "In-vitro antimicrobial activity of Oscimum sanctum leaf extract against pathogens isolated from otitis media infection." IP Int J Med Microbiol Trop Dis 11, no. 3 (2025): 250-256. https://doi.org/10.18231/j.ijmmtd.v.11.i.3.2