In-vitro antimicrobial activity of Oscimum sanctum leaf extract against pathogens isolated from otitis media infection

• Author Details:   
• Jayanti Dash
• Saumya Ranjan Das
• Gopal Krishna Purohit
• Kundan Kumar Sahu
• Subrat Kumar Tripathy
• Debasmita Dubey ^*

Introduction: Otitis media (OM) is the primary contributor to hearing loss in children younger than 5 years, a vital time for the development of speech, language, and cognition. Medications obtained from plants are favoured over synthetic treatments because of their potent pharmacological effects, low toxicity, and cost-effectiveness. O. sanctum has traditionally been used to treat various infections.

Aims and Objective: This study highlights the need for molecular identification of microorganisms linked to OM infection and examining the antimicrobial effects of extracted leaves from O. sanctum on OM infection pathogens.

Materials and Methods: The OM swab samples were processed for microbial identification and antimicrobial susceptibility through the Vitek 2 system, and molecular identification was carried out to identify the pathogens. The five non-polar to polar solvents were used to evaluate the zone of inhibition of all solvents against S. aureus, P. aeruginosa, A. baumannii, K. pneumoniae, and C. albicans. The highest ZOI was reported with methanolic extract against S. aureus, and the best MIC of 0.162 mg/ml was found against C. albicans.

Conclusion: In this study, the O. sanctum methanolic leaves extract has shown good inhibitory activity against OM drug-resistant pathogens.