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- DOI 10.18231/j.ijmmtd.v.11.i.3.10
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CrossMark
Assessing immune parameters among cow urine consumers in diverse Indian settlements: An observational study
- Author Details:
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Nikita Pandit
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Chetana Deoghare *
Chetana Deoghare *
Background: Indigenous cow urine (ICU) is a revered agent in traditional Indian medicine with purported immunomodulatory properties. However, systematic observational data comparing its effects across diverse populations is lacking and further study among the Indian population is recommended.
Aims & Objectives: This study aimed to investigate the association between habitual ICU consumption and immune system parameters in two distinct Indian populations: traditional rural inhabitants and core urban dwellers for their immune body functionalities.
Materials and Methods: A comparative, cross-sectional observational study was conducted in Village Bhadoriya (rural) and Indore city (urban) in Madhya Pradesh. A total of 240 participants were stratified into four groups: Rural Consumers, Rural Non-Consumers, Urban Consumers, and Urban Non-Consumers (n=60 each). Fasting blood samples were analysed for innate and adaptive immune markers, cytokine profiles, and oxidative stress markers. A subset (n=20/group) provided faecal samples for 16S rRNA microbiome sequencing.
Results: Significant positive associations were found between ICU consumption and enhanced immune markers, with more pronounced effects in the rural cohort. Rural consumers showed higher NK cell activity and IgG levels. Urban consumers showed a higher CD4/CD8 ratio and lower IL-6. Both consumer groups exhibited improved antioxidant status (higher SOD/catalase, lower MDA) and rural consumers showed greater gut microbial diversity.
Conclusion: Habitual ICU consumption is associated with favourable immunomodulatory and antioxidant profiles, particularly in a traditional rural setting. These findings support the ethnopharmacological basis for ICU's use and highlight the need for further mechanistic research.
References
- Sharma PV, editor. Caraka Samhita (Text with English Translation). Chaukhambha Orientalia; Varanasi. 2000. https://archive.org/details/CharakaSamhitaTextWithEnglishTanslati onP.V.Sharma
- Murthy KRS. Sushruta Samhita. Chaukhambha Orientalia; Varanasi. 2012.
- Randhawa GK. Cow urine distillate as bioenhancer. J Ayurveda Integr Med. 2010;1(4):240–1.
- Patil PA, Bhosale VV, Girase VP. Review on Gomutra (Cow Urine). Asian J Pharm Res. 2020;10(2):120–2.
- Pandit N, Deoghare C, Chaudhury SK. Experimental ‘in-Vitro’ investigation on bio-chemical constituents, radical scavenging activity, and reducing power assay of cow urine. Pure Appl Chem. 2025;97(5):483–501.
- Paliya N, Deoghare C. Pharmacological potential of cow urine from Red Sindhi breed: In-vitro study on antimicrobial and cytoprotective properties. Indian J Microbiol Res. 2025;12(1):94-9.
- Gulhane H, Nakanekar A, Mahakal N, Bhople S, Salunke A. Gomutra (Cow urine): A multidimensional drug review article. Int J Res Ayurveda Pharm. 2017;8(5):1–6.
- Suharmiati S, Laksono A, Nantabah Z, Kristiana L. Urban-Rural Disparities in Traditional Health Services Utilization in Indonesia: A Cross-sectional Study. J Southwest Jiaotong Univ. 2023;58(3):375–85.
- Hoh JM, Dhanashree B. Antifungal effect of cow's urine distillate on Candida species. J Ayurveda Integr Med. 2017;8(4):233–7.
- Pandit N, Deoghare C, Chaudhury SK, Experimental ‘In-Vitro’ Investigation on Bio-Chemical Constituents, Radical Scavenging Activity, and Reducing Power Assay of Cow Urine. Pure Appl Chem. 2025;97(5).
- Pandit N, Deoghare C, Exploring the pharmacological potential of Red Sindhi Cow urine: An in-vivo approach. IP Int J Med Microbiol Trop Dis. 2024;10(4):324–8.
- Sridharamurthy NB, Manasa MN, Sowmya BA, Anti-Inflammatory Activity of Cow Urine Betel Vine Extract in Rats. World J Res Rev. 2016;2(6):39–42.
- Chauhan RS, Singh BP, Singhal LK. Immunomodulation with Kamdhenu Ark in mice. J Emerg Technol Innov Res. 2001;7(5).
- Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, et al. Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry. 2003;63(1):97–104.
- Paliya N, Deoghare C. Exploring the pharmacological potential of Red Sindhi Cow urine: An in vivo approach. IP Int J Med Microbiol Trop Dis. 2024;10(4):324–8.
- Lehmann AK, Sornes S, Halstensen A. Phagocytosis: measurement by flow cytometry. J Immunol Methods. 2000;243(1-2):229–42.
- Aktas E, Kucuksezer UC, Bilgic S, Erten G, Deniz G. Relationship between CD107a expression and cytotoxic activity. Cell Immunol. 2009;254(2):149–54.
- Maecker HT, McCoy JP, Nuss enblatt R. Standardizing immunophenotyping for high-dimensional data analysis. Nat Rev Immunol. 2012;12(3):191-200.
- Tietz NW. Clinical Guide to Laboratory Tests. 3rd ed. W.B. Saunders Company; 1995.
- Leng SX, McElhaney JE, Walston JD, Xie D, Fedarko NS, Kuchel GA. ELISA and multiplex technologies for cytokine measurement in inflammation and aging research. J Gerontol Biol Sci Med Sci. 2008;63(8):879-84.
- Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351–8.
- Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44(1):276–87.
- Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121–6.
- Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Lozupone CA, Turnbaugh PJ, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci U S A. 2011;108(Suppl 1):4516–22.
- Rinninella E, Raoul P, Cintoni M, Franceschi F, Miggiano GAD, Gasbarrini A, et al. What is the Healthy Gut Microbiota Composition? A Changing Ecosystem across Age, Environment, Diet, and Diseases. Microorganisms. 2019;7(1):14.
- Zheng D, Liwinski T, Elinav E. Interaction between microbiota and immunity in health and disease. Cell Res. 2020;30(6):492–506.
- Pathak ML, Kumar A. Cow urine "ark": A boon for human health. Int J Cow Sci. 2003;1(1):35–8.
- Pandit N, Deoghare C, Chaudhury SK. A review on the green chemistry perspective of multipurpose use of cow urine. Pure Appl Chem. 2024;96(10):1499–522.