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- DOI 10.18231/j.ijmmtd.v.11.i.3.14
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CrossMark
Mycological profile of dermatophytes and their susceptibility pattern at tertiary care centre
- Author Details:
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Jayshree Abhay Pohekar *
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Manjushree Vijay Mulay
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Ashish Deshmukh
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Smita Sitaram Kulkarni
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Shraddha Dinkarrao Naik
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Jyotsna Mishra
Jayshree Abhay Pohekar *
Manjushree Vijay Mulay
Ashish Deshmukh
Smita Sitaram Kulkarni
Shraddha Dinkarrao Naik
Introduction: Dermatophytosis is the commonest contagious fungal infection, commonly known as ringworm infections, more prevalent in tropical & subtropical countries like India. Here heat & moisture helps in promoting its growth. In recent years, the Physician and Microbiologist take more interest in these infections due to various reasons like indiscriminate use of antibiotics, anticancer therapy and immunodeficient diseases like AIDS; as they help in varied clinical presentation of dermathophytic infections. It is observed that resistance to antifungal drugs have started to come up in dermatophytosis. In response to increased incidence of resistance to antifungal drugs, it is necessary to determine the antifungal susceptibility testing (AFST) of isolates to available drugs. In view of above, we have undertaken this study to develop a quick, easy & reliable method of AFST by Agar Based Disk Diffusion (ABDD) method for dermatophytes.
Aim and Objective: To study a quick, easy & reliable method of AFST by ABDD method for isolated dermatophytes.
Materials and Methods: To test AFST of dermatophytic isolates, following antifungal agents were included in the study. Fluconazole, Ketoconazole, Itraconazole, Clotrimazol discs were available commertially (HIMEDIA), and Griseofulvin and Terbinafine discs were prepared in laboratory from powders which were obtained from Siemens Company. Dermatophytic isolates were tested for antifungal susceptibility by ABDD method. Strains were reported as sensitive, intermediate and resistant and were reported to dermatologist for further management.
Results & Conclusion: In this study, we found 46 dermatophytic isolates from skin, nail and hair, in which most common pathogenic dermatophyte isolate was T. mentagrophytes (50%), followed by T. rubrum (43.47%), M. gypseum (4.3%) and T. tonsurans (2.1%). AFST was performed by ABDD method which we found quick and easy. All isolates were 100% sensitive Clotrimazole. All isolates of M.gypseum were sensitive to all drugs. All drugs were effective against T.tonsurans except Fluconazole. Approximately 95% strains were sensitive to Griseofulvin. Approximately 90% strains were sensitive to Terbinafine.
References
- Chandar J. textbook of medical mycology, 4th edition, Mehta publications. 2017
- Lyngdoh CJ, Lyngdoh WV, Choudhury B, Sangma1 KA, Bora I, Khyriem AB, et al. Clinico-mycological profile of dermatophytosis in Meghalaya. Int J Med Public Health. 2013;3(4):254–6.
- Yadav A, Urhekar AD, Mane V, Danu MS, Goel N, Ajit KG. Optimization and isolation of dermatophytes from clinical samples and in vitro antifungal susceptibility testing by disc diffusion method. Res Rev J Microbiol Biotechnol. 2013;2(3):19–34.
- Winn-Jr W, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P, et al. Koneman’s color atlas and textbook of diagnostic microbiology, 6th edition, Lippincott Williams & wilkins.2012.
- Tille PM. Bailey and scott’s, Diagnostic microbiology. 14th edition, Elsevier publication
- White TC, Findley K, Dawson-Jr TL, Scheynius A, Boekhout T, Cuomo CA, et al “Fungi on skin: Dermatophytes and Malassezia. Cold Spring Harb Prospect Med. 2014;4:a019802
- Shalaby MFM, El-din AN, El-Hamd MA. Isolation, identification, and in vitro antifungal susceptibility testing of dermatophytes from clinical samples at sohag university hospital in Egypt. Electron Physician. 2016;8(6):2557–67.
- Anaissie EJ, McGinnis MR, Pfaller MA. Clinical Mycology. 2nd. Churchill Livingstone. 2009.
- S, Mohan K, Malaiyan J, Devasir RS, Meenakshi-Sundaram PR, Selvaraj S. Clinical profile and atypical manifestation of dengue fever cases between 2011 and 2018 in Chennai, India. J Family Med Prim Care. 2020;9(2):1119-1123.
- Banerjee S, Khan K, Mandal P, Mallick SK. Current mycological profile of dermatophytosis in a tertiary care set up in North Bengal. J Pak Assoc Dermatol. 2015;25(1):35–9.
- Nilekar SL, Kulkarni VL. Dermatophytosis in and around Ambajogai. IOSR J Dent Med Sci. 2015;14(10):37–41.
- Matnani G, Roy I, Gandham N, Mandal A, Ujagare M, Jadhav SV. Identification and antifungal susceptibility testing of fungal infections in clinical samples of suspected superficial fungal infections. Int J Med Clin Res. 20212;3(12):215–20.
- Kumar K, Kindo AJ, Kalyani J, Anandan S. Clinico-mycological profile of dermatophytic skin infections in a tertiary care center a cross sectional study. Sri Ramchandra J Med. 2007;1(2):12–4.
- Rathod PG, Shaikh NK, Ingole KV, Mundhada SG, Chakote SM. Prevalence of dermatophytes in a tertiary care center of Solapur, Maharashtra. J Krishna Inst Med Sci Univ. 2016;5(3):26–34.
- Doddamani PV, Harshan KH, Kanta RC, Gangane R, Sunil KB. Isolation, identification and prevalence of dermatophytes in tertiary care hospital in Gulbarga district. People’s J Sci Res. 2013;6(2):10–
- Panday A, Panday M. Isolation and characterization of dermatophytes with tinea infection in Gwalior (MP) India. Int J Pharm Sci Invent. 2013;2(2):5–8.
- Dogra S, Shaw D, Rudramuethy S. Antifungal Drug Susceptibility Testing of Dermatophytes Laboratory Findings to Clinical Implications. Indian Dernatol Online J. 2019;10(3):225–33.
- Basavarajappa MS, Madhusudan SK, Rudrappa RM, Reddy SK. Evaluation of antifungal susceptibility pattern of dermatophytes isolated in tertiary care hospital. Natl J Physiol Pharm Pharmacol. 2022;12(10):1565–71.
- Agrawal RK, Gupta S, Mittal G, Khan F, Roy S, Agarwal A. Antifungal susceptibility testing of dermatophytes by agar based disk diffusion method. Int J Curr Microbiol Appl Sci. 2015;4(3):430–6.
- Das S, De A, Saha R, Sharma N, Khema M, Singh S, et al. The Current Indian Epidemic of Dermatophytosis: A Study on Causative Agents and Sensitivity Pattern. Indian J Dermatol. 2020;65(2):118–
- Gupta S, Agarwal RK, Mittal G, Roy S, Khan F, Agarwalt A. Comparision of broth dilution and disk diffusion method for susceptibility testing of dermatophytes. Int J Curr Microbiol Appl Sci. 2015;4(5):24–33.
- Esteban A, Abarca ML, Cabanes FJ. Comparison of disk diffusion method and broth dilution method for antifungal susceptibility testing of dermatophytes. Med Mycol. 2005;43(1):61–6.
- Pakshir K, Bahaedinie L, Rezaei Z, Sodaifi M, Zomorodian K. In vitro activity of six antifungal drugs against clinically important dermatophytes. Jundishapur J Microbiol. 2009;2(4):158–63.
- Walke HR, Gaikwad AA, Palekar SS, Clinico-mycological profile of dermatophytosis in patients attending dermatology OPD in tertiary care hospital, India. Int J Curr Microbiol Appl Sci. 2014;3(10):432–40.
- Gupta CM, Dhanvijay A, Kiran T, Nema S. Current trends of Clinicomycological profile of dermatophytes in Central India. IOSR J Dent Med Sci. 2004;13(10):23–6.
- Sowmya N, Appalaraju B, Srinivas CR, Surendran P. Antifungal susceptibility testing for dermatophytes isolated from clinical samples by broth dilution method in tertiary care hospital. J Med Res. 2015;1(2):64–7.
- Barros MES, Santos DA, Hamdan JS. Evaluation of susceptibility of Trichophyton mentagrophyte and Trichophyton rubrum clinical isolates to antifungal drugs using modified CLSI microbroth dilution method (M38-A). J Med Microbiol. 2007;56(Pt 4):514–8.
- Ghannoum MA, Arthington-Skaggs B, Chaturvedi V, Espinel- Ingroff A, Pfaller MA, Rennie R, et al. Interlaboratory study of Quality Control Isolates for a Broth Microdilution Method (Modified CLSI M 38-A) for Testing Susceptibility of Dermatophytes to Antifungals. J Clin Microbiol. 2006;44(12):4353.
- Nweze EI, Mukharjee PK, Ghannoum MA. Agar-Based disk diffusion for susceptibility testing of dermatophytes. J Clin Microbiol. 2010;48(10):3750–2.
- Singh J, Zaman M, Gupta AK. Evaluation of microdilution and disk diffusion methods for antifungal susceptibility testing of dermatophytes. Med Mycol. 2007;45(7):595–602.
- Anantnarayanan R, Paniker’s CKJ. Textbook of microbiology. 10th edition, Universities press. 2017. 322 Pohekar et al. / IP International Journal of Medical Microbiology and Tropical Diseases 2025;11(3):316-322.
- Zaki SM, Eikholy IM, Mohamed SS, Wasfy WM. In-Vitro susceptibility testing of dermatophytes isolated in Cairo, Egypta against eight antifungal agents by north microdilution and disk diffusion methods. J Basic Appl Mycol (Egypt). 2015;6:9–15
- Ansari HQF, Patel MB, Siddiqui JAW. Epidemiology and In Vitro Antifungal Susceptibility Testing of Dermatophytes in Hyderabad, India. Int J Adv Res. 2014;2(2):553–60.